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http://hdl.handle.net/20.500.12701/2146
Полная запись метаданных
Поле DC | Значение | Язык |
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dc.contributor.author | Nikitina, T. V. | - |
dc.contributor.author | Kashevarova, A. A. | - |
dc.contributor.author | Gridina, M. M. | - |
dc.contributor.author | Lopatkina, M. E. | - |
dc.contributor.author | Khabarova, A. A. | - |
dc.contributor.author | Yakovleva, Yu. S. | - |
dc.contributor.author | Menzorov, A. G. | - |
dc.contributor.author | Minina, Yu. A. | - |
dc.contributor.author | Pristyazhnyuk, I. E. | - |
dc.contributor.author | Vasilyev, S. A. | - |
dc.contributor.author | Fedotov, D. A. | - |
dc.contributor.author | Serov, O. L. | - |
dc.contributor.author | Lebedev, I. N. | - |
dc.date.accessioned | 2022-06-25T06:19:25Z | - |
dc.date.available | 2022-06-25T06:19:25Z | - |
dc.date.issued | 2021-02-22 | - |
dc.identifier.uri | https://doi.org/10.1038/s41598-021-83399-3 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.12701/2146 | - |
dc.description.abstract | Human ring chromosomes are often unstable during mitosis, and daughter cells can be partially or completely aneuploid. We studied the mitotic stability of four ring chromosomes, 8, 13, 18, and 22, in long-term cultures of skin fibroblasts and induced pluripotent stem cells (iPSCs) by GTG karyotyping and aCGH. Ring chromosome loss and secondary aberrations were observed in all fibroblast cultures except for r(18). We found monosomy, fragmentation, and translocation of indexed chromosomes. In iPSCs, aCGH revealed striking differences in mitotic stability both between iPSC lines with different rings and, in some cases, between cell lines with the same ring chromosome. We registered the spontaneous rescue of karyotype 46,XY,r(8) to 46,XY in all six iPSC lines through ring chromosome loss and intact homologue duplication with isoUPD(8)pat occurrence, as proven by SNP genotype distribution analysis. In iPSCs with other ring chromosomes, karyotype correction was not observed. Our results suggest that spontaneous correction of the karyotype with ring chromosomes in iPSCs is not universal and that pluripotency is compatible with a wide range of derivative karyotypes. We conclude that marked variability in the frequency of secondary rearrangements exists in both fibroblast and iPSC cultures, expanding the clinical significance of the constitutional ring chromosome. | ru_RU |
dc.language.iso | en | ru_RU |
dc.publisher | Springer Nature Limited | ru_RU |
dc.relation.ispartofseries | Scientific Reports;Volume 11, Issue 4325 | - |
dc.subject | Stem cells | ru_RU |
dc.subject | Genetics | ru_RU |
dc.title | Complex biology of constitutional ring chromosomes structure and (in)stability revealed by somatic cell reprogramming | ru_RU |
dc.type | Article | ru_RU |
Располагается в коллекциях: | Scientific Reports |
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Файл | Описание | Размер | Формат | |
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10.1038s41598-021-83399-3.pdf | 7,71 MB | Adobe PDF | Просмотреть/Открыть |
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