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dc.contributor.authorVorobyeva, Anzhelika-
dc.contributor.authorBezverkhniaia, Ekaterina-
dc.contributor.authorKonovalova, Elena-
dc.contributor.authorSchulga, Alexey-
dc.contributor.authorGarousi, Javad-
dc.contributor.authorVorontsova, Olga-
dc.contributor.authorAbouzayed, Ayman-
dc.contributor.authorOrlova, Anna-
dc.contributor.authorDeyev, Sergey-
dc.contributor.authorTolmachev, Vladimir-
dc.date.accessioned2022-04-13T09:43:58Z-
dc.date.available2022-04-13T09:43:58Z-
dc.date.issued2020-10-14-
dc.identifier.urihttps://doi.org/10.3390/molecules25204719-
dc.identifier.urihttp://hdl.handle.net/20.500.12701/1975-
dc.description.abstractEfficient treatment of disseminated triple-negative breast cancer (TNBC) remains an unmet clinical need. The epithelial cell adhesion molecule (EpCAM) is often overexpressed on the surface of TNBC cells, which makes EpCAM a potential therapeutic target. Radionuclide molecular imaging of EpCAM expression might permit selection of patients for EpCAM-targeting therapies. In this study, we evaluated a scaffold protein, designed ankyrin repeat protein (DARPin) Ec1, for imaging of EpCAM in TNBC. DARPin Ec1 was labeled with a non-residualizing [125I]I-para-iodobenzoate (PIB) label and a residualizing [99mTc]Tc(CO)3 label. Both imaging probes retained high binding specificity and affinity to EpCAM-expressing MDA-MB-468 TNBC cells after labeling. Internalization studies showed that Ec1 was retained on the surface of MDA-MB-468 cells to a high degree up to 24 h. Biodistribution in Balb/c nu/nu mice bearing MDA-MB-468 xenografts demonstrated specific uptake of both [125I]I-PIB-Ec1 and [99mTc]Tc(CO)3-Ec1 in TNBC tumors. [125I]I-PIB-Ec1 had appreciably lower uptake in normal organs compared with [99mTc]Tc(CO)3-Ec1, which resulted in significantly (p < 0.05) higher tumor-to-organ ratios. The biodistribution data were confirmed by micro-Single-Photon Emission Computed Tomography/Computed Tomography (microSPECT/CT) imaging. In conclusion, an indirectly radioiodinated Ec1 is the preferable probe for imaging of EpCAM in TNBC.ru_RU
dc.language.isoenru_RU
dc.publisherMDPIru_RU
dc.relation.ispartofseriesMolecules;Volume 25, Issue 20-
dc.subjectEpCAMru_RU
dc.subjectradionuclideru_RU
dc.subjectmolecular imagingru_RU
dc.subjectSPECTru_RU
dc.subjectiodineru_RU
dc.subjectPIBru_RU
dc.subjectbreastru_RU
dc.subjectcancerru_RU
dc.titleRadionuclide Molecular Imaging of EpCAM Expression in Triple-Negative Breast Cancer Using the Scaffold Protein DARPin Ec1ru_RU
dc.typeArticleru_RU
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