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dc.contributor.authorTetenev, Konstantin-
dc.contributor.authorCloutier, Mary E.-
dc.contributor.authorvon Reyn, Jessica A.-
dc.contributor.authorAther, Jennifer L.-
dc.contributor.authorCandon, James-
dc.contributor.authorAllen, Gilman B.-
dc.date.accessioned2022-04-01T08:10:45Z-
dc.date.available2022-04-01T08:10:45Z-
dc.date.issued2015-11-15-
dc.identifier.urihttps://doi.org/10.1152/ajplung.00197.2014-
dc.identifier.urihttp://hdl.handle.net/20.500.12701/1811-
dc.description.abstractAspiration is a common cause of lung injury, but it is unclear why some cases are self-limited while others progress to acute respiratory distress syndrome (ARDS). Sporadic exposure to more than one insult could account for this variable progression. We investigated whether synergy between airway acid and endotoxin (LPS) amplifies injury severity in mice and whether LPS levels in human patients could corroborate our experimental findings. C57BL/6 mice aspirated acid (pH 1.3) or normal saline (NS), followed by LPS aerosol or nothing. Bronchoalveolar lavage fluid (BALF) was obtained 2 to 49 h later. Mice were injected with FITC-dextran 25 h after aspiration and connected to a ventilator, and lung elastance (H) measured periodically following deep inflation (DI). Endotracheal and gastric aspirates were also collected from patients in the intensive care unit and assayed for pH and LPS. Lung instability (ΔH following DI) and pressure-volume hysteresis in acid- or LPS-exposed mice was greater than in controls but markedly greater in the combined acid/LPS group. BALF neutrophils, cytokines, protein, and FITC-dextran in the acid/LPS mice were geometrically higher than all other groups. BALF from acid-only mice markedly amplified LPS-induced TNF-α production in cultured macrophages. Human subjects had variable endotracheal LPS levels with the highest burden in those at higher risk of aspiration. Acid aspiration amplifies LPS signaling in mice to disrupt barrier function and lung mechanics in synergy. High variation in airway LPS and greater airway LPS burden in patients at higher risk of aspiration could help explain the sporadic progression of aspiration to ARDS.ru_RU
dc.language.isoenru_RU
dc.publisherAmerican Physiological Societyru_RU
dc.relation.ispartofseriesAmerican Journal of Physiology Lung Cellular and Molecular Physiology;Volume 309, Issue 10-
dc.subjectlung mechanicsru_RU
dc.subjectrespiratory impedanceru_RU
dc.subjectforced oscillationsru_RU
dc.subjectacid aspirationru_RU
dc.subjectendotoxinru_RU
dc.subjectacute lung injuryru_RU
dc.subjectARDSru_RU
dc.titleSynergy between acid and endotoxin in an experimental model of aspiration-related lung injury progressionru_RU
dc.typeArticleru_RU
Располагается в коллекциях:American Journal of Physiology-Lung Cellular and Molecular Physiology

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